Heat map showing newly identified miRNA expression patterns in 10 tissues (liver, gill, head kidney, spleen, heart, brain, muscle, stomach, intestines and skin) measured by stem-loop RT-PCR.

<p>Relative expression levels of the 45 novel miRNAs were measured in terms of threshold cycle value (Ct) and were normalized to 5S rRNA. The expression data were analyzed by hierarchical clustering for both tissues and genes.