miRNAs identified by the MAs during WT skeletal muscle development.

<p>(A) Up (orange) and down (blue) regulated DEGs identified by the MAs for E18.5 vs. E14.5 (E14.5 is control) taking part in the Wiki pathway “Mirs in Muscle Cell Differentiation Homo sapiens (2012)”. DEGs regulated only in WT samples from E14.5 to E18.5 are shown in bold. (B) A heat map of all miRNAs, found to be differentially regulated at E18.5 compared to E14.5 in WT samples. Each row represents one biological replicate. miRNAs, found to be differentially regulated from E14.5 to E18.5 also in RYR1^-/- samples, are underlined in yellow, in the Ca_v1.1^-/- samples in red, and in both RYR1^-/- and Ca_v1.1^-/- samples in yellow and red, respectively. The heatmap was generated from the MAs intensity levels of each of the Mir genes via ClustVis [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194428#pone.0194428.ref032" target="_blank">32</a>].