Next-generation sequencing analysis of miRNA expression in control and FSHD myogenesis.

Veronica Colangelo
Stéphanie François
Giulia Soldà
Raffaella Picco
Francesca Roma
Enrico Ginelli
Raffaella Meneveri

Open link

DOI

10.1371/journal.pone.0108411

Publisher

Public Library of Science (PLoS)

Journal

issn:1932-6203

Abstract

Emerging evidence has demonstrated that miRNA sequences can regulate skeletal myogenesis by controlling the process of myoblast proliferation and differentiation. However, at present a deep analysis of miRNA expression in control and FSHD myoblasts during differentiation has not yet been derived. To close this gap, we used a next-generation sequencing (NGS) approach applied to in vitro myogenesis. Furthermore, to minimize sample genetic heterogeneity and muscle-type specific patterns of gene expression, miRNA profiling from NGS data was filtered with FC ≥ 4 (log(2)FC ≥ 2) and p-value<0.05, and its validation was derived by qRT-PCR on myoblasts from seven muscle districts. In particular, control myogenesis showed the modulation of 38 miRNAs,...