Altered microRNA expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.
- Ji-Feng Xu
- Guang-Hai Yang
- Xiao-Hong Pan
- Shui-Jun Zhang
- Chen Zhao
- Bin-Song Qiu
- Hai-Feng Gu
- Jian-Fei Hong
- Li Cao
- Yu Chen
- Bing Xia
- Qin Bi
- Ya-Ping Wang
DOIAbstract
The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal miRNAs isolated from human bone marrow-derived mesenchymal stem cells (BMSCs) culture were profiled using miRNA arrays containing probes for 894 human matured miRNAs. Seventy-nine miRNAs (∼8.84%) could be detected in exosomes isolated from BMSC culture supernatants when normalized to endogenous control genes RNU44. Among them, nine exosomal miRNAs were up regulated and 4 miRNAs were under regulated significantly (Relative fold>2, p<0.05) when compared with the values at 0 day with maximum changes at 1 to 7 days. Five miRNAs (miR-199b, miR-218, miR-148a, miR-135b, and miR-221) were further validated and differentially expressed in the individ...
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