Bcl2-ARE^flox/flox x mb1^cre mice have a reduced numbers of FO B cells in the periphery.

<p><b>A</b>, Flow cytometry analysis of FO B cells (CD19⁺ CD93^- CD23⁺ CD21⁺ cells), MZ B cells (CD19⁺ CD93^- CD23^low CD21^high cells) and transitional B cells (T1: CD19⁺ CD93⁺ IgM⁺ CD23^- cells; T2: CD19⁺ CD93⁺ IgM⁺ CD23⁺ cells; and T3: CD19⁺ CD93⁺ IgM^low CD23⁺ cells cells) in spleens from Bcl2-ARE^flox/flox x mb1^wt (flox/flox) and Bcl2-ARE^flox/flox x mb1^cre (∆/∆) mice. N = 6/7 mice per genotype. The mean percentage of cells in each gate is shown in each representative plot. <b>B</b>, Quantification of the number of total splenocytes, FO B cells, MZ B cells, and transitional B cells, defined as described above. <b>C</b>, Analysis of CD19⁺ B cells and CD4⁺ T cells in peripheral LN (inguinal, brachial and axillary LNs were pooled together for the analysis). The mean percentage of cells in each gate is shown in the plots. N = 5–7 mice per genotype. <b>D</b>, Quantitation of the number of CD19⁺ B cells and CD4⁺ T cells. A Mann-Whitney test was performed for statistical analysis of the data. P values are shown for each cell population.