Reduced numbers of B cells in the <i>Gnai2</i>^fl/fl<i>mb1-cre</i> mice and DKO mice.

<p>A. Flow cytometric analysis of lymphocytes in the spleen and LNs of control and mutant mice. Pooled results from analyzing cells prepared from spleens and LNs from 9 <i>Gnai2</i>^fl/fl, 8 <i>Gnai2 </i>^fl/fl<i>mb1-cre</i>, 3 Gnai3^-/-, and 3 DKO mice. B. Flow cytometric analysis of lymphocytes in Peyer’s patches and bone marrow of control and mutant mice. Pooled results from analyzing cells from Peyer’s patches and bone marrow from the same mice used in part A. ND- not detected. C. Flow cytometric analysis of B cell populations in the spleen of control and mutant mice. Pooled results from analyzing cells from the spleens prepared from the same mice used in part A. D. Flow cytometric analysis of immature B cell populations in the spleen. The number of pre-B and T0 B cells pooled and compared to T1 B cells. Data is mean +/- SEM. Statistical significance determined by 2 way ANOVA. ** p<0.001, * p<0.05.