Phenotypic analysis of each B cell subset isolated from Balb/c mice.

<p>Naïve B cells from the SPL, PerC, and liver were stained with various combinations of mAbs directed against IgM, CD19, CD21, CD11b, CD5, PD-L1, PD-L2, FasL, TRAIL, CD80, CD86, and MHC class II, and were analyzed by performing FCM. Then, these cells were divided into the following subsets: CD21^intIgM^int follicular (FO) B cells, CD21^highIgM^high marginal zone (MZ) B cells, and CD21^-/lowIgM^high B-0 cells from the SPL, and CD11b⁺CD5⁺ B-1a cells, CD11b⁺CD5^- B-1b, and CD11b^-CD5^- B-2 cells from the PerC and liver (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0178765#pone.0178765.s002" target="_blank">S2 Fig</a>). (A) Histograms of PD-L1, PD-L2, FasL, TRAIL, CD80, CD86, and MHC class II on each B cell subset by performing FCM analysis. (B) Percentage (mean ± SEM) expression of each membrane marker expressed on each B cell subset. *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001, and ****<i>p</i> < 0.00001(Student’s <i>t</i>-test). Data are representative of three experiments with three mice per group.