Analysis of B cells development in spleen and PEC of transgenic mice.

<p>(A) The phenotype of B cells in the spleen of TgV_H3B4I and TgV_H/L3B4 mice. CD19⁺ or IgM^a+ splenic B cells from indicated mice were analyzed for the expression of CD21 and CD23 by FACS. Numbers next to each gate indicate the percentage of cells in that gate in total CD19⁺ or IgM^a+ cells. At least 7 mice from each genotype were analyzed. (B) Spleen sections from the indicated mice were stained with anti-MOMA1-FITC and anti-B220-Biotin (left panels) or anti-IgM^a-biotin (right panel) followed by streptavidin-Cy3, and acquired using fluorescence microscopy. Follicular (Fo) areas around PALS are shown and MZ is indicated with arrow. The original magnitude was ×200 or ×400 as indicated. (C) CD19⁺ or IgM^a+ B cells in PEC of indicated mice were evaluated for the expression of CD5 by FACS. At least 5 mice from each genotype were analyzed. Numbers next to each gate indicate the percentage of cells in that gate in total CD19⁺ or IgM^a+ cells.