B cell analysis in pre-tumoral mice.

<p>A) Splenic cells of the indicated groups were stained with antibodies recognizing CD19 and IgM. Proportion of CD19+ IgM− cells is reported. B) Mice of the indicated groups were injected intraperitoneally with BrdU 2 hours before their sacrifice. Splenic cells were incubated with antibodies against CD19, IgM and BrdU. The plot indicates proportion of BrdU positive cells in the CD19+/IgM− subpopulation. Representative dot plots of the CD19⁺ IgM⁻ cells are on the right. C) Splenic cells were incubated with antibodies against CD19, IgM and cleaved Caspase 3. Percentage of cl-Casp3 positive cells in the CD19⁺/IgM⁺ and in the CD19⁺/IgM⁻ population is reported (* p<0.05 <i>E</i>μ<i>Myc Prep1^+/+</i> CD19⁺/IgM⁻ vs. <i>E</i>μ<i>Myc Prep1</i>^+/− CD19⁺/IgM⁻). Representative dot plots of the CD19+/IgM⁻-gated cells are on the right.