Most thymic B cells in <i>the Foxn1</i>^<i>lacZ</i> thymus have an immature phenotype.

<p><b>(A).</b> Representative profiles of CD19 and B220 staining (left panels) and CD24 and CD43 (right panels) on gated B cells in thymocytes from +/Z, Z/Z, and Z/N mice as indicated to the left. <b>(B,C)</b> Same analysis of splenocytes (B) and BM cells (C) from +/Z mice. <b>(D).</b> The ratio of CD19^loB220^lo immature B (im-B) to CD19^hiB220^hi mature B (m-B) cells in thymus, spleen and BM cells. <b>(E-F).</b> The percentage of CD43^-/loCD24^lo (E) and CD43^lo/hiCD24^hi (F) B subsets in total thymic B cells. <b>(G).</b> Histograms show overlapping profiles of Ly51, CD93, CD25, MHCII or IgM staining on gated thymic B cells from +/Z, Z/Z and Z/N mice. All assays were performed at day 30. Data are representative of five individual experiments, (+/Z: n = 7, Z/Z: n = 5, Z/N: n = 8). One-way ANOVA: *P <0.05, **P <0.01, ***P <0.001, ****P<0.0001. ns: not significant. Bars indicate means ± SEM.