SIRT1 is localized in both the cytoplasm and the nucleus in HUVECs.

<p>SIRT1 was stained green with an anti-SIRT1 antibody. The nuclei were stained with DAPI, and the merge represents the combined image of SIRT1 fluorescence and nuclear staining. The cells were pretreated with different concentrations of resveratrol or transfected with SIRT1 siRNA, followed by TNF-α stimulation for 24 h. Representative images are shown to indicate the impact of resveratrol on the subcellular localization of SIRT1. The same results were obtained in three independent experiments. Magnification, 200×.