Effects of SIRT1 knockdown on functions of SIRT1 modulators.

<p>(A) Immunoblot analysis for SIRT1 in C2C12 cells transfected with control (Ctrl)- or SIRT1-siRNA. (B) Representative images of CM-H₂DCFDA fluorescence. C2C12 cells transfected with control- (Ctrl-) or SIRT1-siRNA were treated with antimycin A (AA) (100 µM) for 3 hours after pretreatment with vehicle or resveratrol (RSV, 10 µM) for 6 hours. Scale bar: 10 µm. (C) Quantification of CM-H₂DCFDA (DCF) fluorescence. (D and E) Quantification of cells with condensed nuclei. C2C12 cells transfected with control- (Ctrl-) or SIRT1-siRNA were treated with AA (200 µM) for 24 hours after pretreatment with vehicle (Ctrl), 10 µM RSV, or 60 µM splitomicin (SP) for 3 hours. Data are from three independent experiments. **p<0.01, *p<0.05. a.u. = arbitrary unit. n.s. = not significant.