Effects of SIRT1 modulators on H₂O₂-induced ROS generation and apoptosis.

<p>C2C12 cells were treated with 50 µM H₂O₂ for 24 hours after their pretreatment with vehicle (Ctrl), 5 mM nicotinamide (NA), 60 µM splitomicin (SP), 10 µM resveratrol (RSV), or 1 mM NAD⁺ for 3 hours. (A) Intracellular levels of reactive oxygen species (ROS) were detected by CM-H₂DCFDA (DCF). Data are from three independent experiments. (B and C) Cell death was analyzed by nuclear condensation (B), or by immunostaining for cleaved (active) caspase-3 (C). Data in each panel are from three independent experiments. **p<0.01, *p<0.05. a.u. = arbitrary unit.