A. Analysis of the effects of SIRT1-knockdown in N2aSwe cells. Proteins (30 μg) from a negative control and SIRT1- knockdown samples were loaded onto 10 ~ 15% SDS-polyacrylamide gels. In N2aSwe cells transfected with 200 nM of SIRT1 siRNA, SIRT1 protein levels were at ~ 40% of the level in negative controls (A). Cilostazol failed to elevate the expressions of SIRT1 (B), beclin1 (C), Atg5 (D), and (E) LC3 in SIRT1-siRNA treated N2a cells, as contrasted to the levels of all four in the negative control cells.

<p>Results are the means ± SDs of 4 experiments. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001 vs. DMSO. ^††<i>P</i> < 0.01 vs. cilostazol alone (CSZ, 10 μM).