SirT1 Induces Changes in Contractile Proteins Indicative of Differentiation.

<p>A, HuVSMC treated with increasing concentrations of resveratrol for 24 hours before western blot assessment with primary antibodies as indicated. Bar graphs represent densitometric quantification. N = 9 experiments. Probability values are indicated above bars: * p<0.05, **p<0.01, ***p<0.001 versus control. B, HuVSMC were transfected with control small interfering (si) RNA or SirT1 siRNA for 48 hours and then assessed by western blot with primary antibodies as indicated. Bar graphs represent densitometric quantification. N = 2 experiments, in duplicate. Probability values are indicated above bars: * p<0.05, **p<0.01 versus control. C, HuVSMC were transfected with control or SirT1 plasmid for 48 hours and then assessed by western blot with primary antibodies as indicated. At higher exposures, endogenous SirT1 is present in this experiment (data not shown). Bar graphs represent densitometric quantification. N = 3 experiments. Probability values are indicated above bars: **p<0.01 versus control.