AMPK Inhibits the mTORC1 Pathway to Induce Differentiation.

<p>A, HuVSMC treated with increasing concentrations of resveratrol for 24 hours prior to western blot assessment with primary antibodies as indicated. Bar graphs represent densitometric quantification. Samples have been normalized to GAPDH and then fold change from normalized values have been graphed. N = 5 experiments. Probability values are indicated above bars: * p<0.05 versus control. B, HuVSMC treated with increasing concentrations of resveratrol for 24 hours prior to western blot assessment of primary antibodies as indicated. Bar graphs represent densitometric quantification. Samples have been normalized to GAPDH and then fold change from normalized values have been graphed. N = 3 experiments. Probability values are indicated above bars: * p<0.05, *** p<0.001 versus control. C, HuVSMC were transfected with control siRNA or AMPK α1 siRNA for 24 hours, prior to treatment with 30 µM resveratrol or control. Twenty-four hours after treatment, cells were collected and assessed by western blot with primary antibodies as indicated. Bar graphs represent densitometric quantification. N = 4 experiments. Probability values are indicated above bars: * p<0.05, **p<0.01, *** p<0.001 versus control or