Inhibition of SIRT1 reverses the resveratrol-imposed suppression of CD40 expression in TNF-α-treated HUVECs.

<p><b>(A)</b> HUVECs were either transfected with specific siRNA against SIRT1, Nc-siRNA (scrambled siRNA), or Ex527 with Lipofectamine for 24 h. Whole cell lysates were subjected to western blot analysis. <b>(B)</b> Densitometric analysis of the blots shown in A. **<i>p</i> < 0.05 vs. Nc-siRNA group. <b>(C)</b> RT-qPCR was performed as described in the Materials and Methods section. TNF-α, 10 μg/L; resveratrol, 10 μM; Ex527, 10 μM; PDTC, 20 μM. The results are expressed as the mean ± SEM from three independent assays, each carried out in triplicate. **<i>p</i> < 0.01 vs. control; ##<i>p</i> < 0.01 vs. TNF-α alone; ++<i>p</i> < 0.01 vs. TNF-α + resveratrol.