Inhibition of SIRT1 reduces the antagonistic effects of resveratrol on the TNF-α-induced increase in CD40 expression in HUVECs.

<p>HUVECs were pretreated with 10 μM resveratrol in the presence or absence of Ex527 before TNF-α stimulation. PDTC and SB203580 were also applied to explore the involvement of the respective signaling pathways. <b>(A)</b> Representative images of the flow cytometry experiment show each experimental condition and the cell percentages in each gate from independent experiments. More than 5 × 10³ cells were detected. <b>(B)</b> The mean fluorescence intensity of CD40 was calculated. **<i>p</i> < 0.01 vs. control; #<i>p</i> < 0.05 and ##<i>p</i> < 0.01 vs. TNF-α alone; +<i>p</i> < 0.05 and ++<i>p</i> < 0.01 vs. TNF-α + resveratrol.