Resveratrol Inhibited inflammation in a Sirt1 dependent manner.

<p><b>A.</b> Western blotting analysis shows Sirt1 siRNA (20 nM) transfection for 72 hours strikingly blocked Sirt1 expression, compared to control (Cont) siRNA (20 nM) or DDW transfection. <b>B.</b> After Sirt1 or control siRNA transfection for 72 h, cells were h pretreated with resveratrol (50 nM) in the presence of TNF-α (10 ng/ml) treatment for 24 h, MMP-9 expression in the medium were examined by gelatin zymography. <b>C.</b> Cells were processed as (B) for 6 h, real-time RT-PCR analysis was used to examine mRNA expression of MMP-9, IL-6, iNOS (n = 3 per group, *<i>p</i><0.05 vs. Sirt1 siRNA-; **<i>p</i><0.01 vs. Sirt1 siRNA-).