Resveratrol decreases activated-STAT1 levels in the nucleus of cytokine-stimulated HT-29 cells more efficiently than 5-ASA.

<p>Cells were pre-incubated with 25 µM Resv or 500 µM 5-ASA or both (25 µM Resv plus 500 µM 5-ASA) and then exposed to a combination of cytokines for 30 minutes. The levels of Tyr701 phospho-STAT1 were analyzed in nuclear extracts by Western blotting (A), as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109048#s2" target="_blank">Materials and Methods</a>” and expressed as percentage of cytokine-stimulated cells. Values are mean ± SEM of at least three independent experiments, each one in duplicate. ^***<i>P</i><0.001 vs Control and ^##<i>P</i><0.01 vs Cytokines. (B) Representative confocal microscopy pictures of non-stimulated, cytokine-stimulated and Resv pre-incubated HT-29 cells. Simultaneous DNA labelling with Hoechst was performed to visualize the nuclear compartments.