Resveratrol inhibits cytokine-induced oxidative stress in a greater extent than 5-ASA, in HT-29 cells.

<p>Cells were pre-incubated with 25 µM Resv or 500 µM 5-ASA or both (25 µM Resv plus 500 µM 5-ASA) for 1 hour and then exposed to a combination of cytokines for 16 hours. Reactive species production was measured after 16 hours of incubation with cytokines, by oxidation of the probe dichlorodihydrofluorescein, expressed in terms of fluorescence intensity relative to cytokine-stimulated HT-29 cells. A time-course of HT-29 reactive species production following cytokine challenge is presented on the top and representative images obtained by fluorescence microscopy (400×) of cells at 16 hours after cytokine treatment, in the absence or presence of 25 µM Resv and/or 500 µM 5-ASA are presented on the right. Values are mean ± SEM of at least three independent experiments, each one in duplicate. ^**<i>P</i><0.01 vs Control, ^#<i>P</i><0.05, ^##<i>P</i><0.01 vs Cytokines and ^$<i>P</i><0.05 vs ASA plus Cytokines.