Resveratrol does not prevent IkB-α degradation induced by cytokines, in HT-29 cells.

<p>Cells were pre-incubated with 25 µM Resv or 500 µM 5-ASA or both (25 µM Resv plus 500 µM 5-ASA) and then exposed to a combination of cytokines for 30 minutes. IkB-α degradation was analyzed in cytoplasmic extracts by Western blotting, as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109048#s2" target="_blank">Materials and Methods</a>”, and expressed as percentage of control cells, i.e. cells not treated. Values are mean ± SEM of at least three independent experiments, each one in duplicate. ^***<i>P</i><0.001 vs Control.