Inhibition of resveratrol on the phosphorylation of IKBα, NF-κB p50, and NF-κB p65.

<p>Western blot analyses of cell lysates of RD cells pre-incubated with or without resveratrol (30 μg/ml) for 1.5 h, and then infected with EV71 (MOI = 2) at 0, 4, 8, 12 and 24 h p.i. were conducted using antibodies against total or phosphorylated IKBα <b>(A and B)</b>, NF-κB p50 <b>(C and D)</b>, and NF-κB p65 <b>(E and F),</b> and used GAPDH as the control. The intensity of phosphorylated and total protein band was quantitated by densitometric analysis and normalized to GAPDH. The data were expressed as mean ± SE from three independent experiments and analyzed by One-way ANOVA followed by a Dunnett's Multiple Comparison Test (*<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001).