Myeloid cell (<i>LysM</i>⁺) expression of Map3k8 regulates T_H2-mediated immunopathology and fibrosis following <i>S</i>. <i>mansoni</i> infection.

<p><i>LysM</i>^<i>Cre</i><i>Map3k8</i>^<i>+/+</i> and <i>LysM</i>^<i>Cre</i><i>Map3k8</i>^<i>fl/fl</i> mice were infected percutenously with 50 <i>S</i>. <i>mansoni</i> cercariae and analysed at 8 weeks post-infection. A–B) Perfused tissue was fixed and embedded in paraffin before sectioning and staining with Masson’s trichrome. Scale bar is 200μm. C) Granuloma size was determined from 10–20 individual granulomas per sample measured using Image J. D) Intestinal pathology score, as described in methods. E) Expression of <i>Col3</i> and <i>Col6</i> was determined from RNA extracted from liver. Data is expressed relative to HPRT and presented as a fold-change relative in infected WT mice. F) Hydroxyproline was quantified in liver tissue from naïve and infected animals. G) Mesenteric lymph node cells were re-stimulated with anti-CD3 for 3 days. Cytokines were measured in supernatants, by ELISA. All experiments are representative of 2–3 independent experiments with 5–10 mice/genotype. * p< 0.05 as assessed by two-tailed Mann-Whitney test.