Diminished myeloid output in <i>Fzd6</i>^-/- mice correlates with a reduced parasite burden during the chronic phase of infection.

<p><b>(A)</b> Analysis of bone marrow myeloid subsets infected <i>Fzd6</i>^-/- (KO) and <i>Fzd6</i>^+/+ (WT) mice. Mean percentage for each cell subset is indicated within flow cytometry plots. Graphs show numbers of granulocytes and monocytes on day 14 and 28. See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006422#ppat.1006422.s004" target="_blank">S4</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006422#ppat.1006422.s006" target="_blank">S6</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006422#ppat.1006422.s007" target="_blank">S7</a> Figs. <b>(B)</b> Numbers within flow cytometry plots indicate mean percentage of Ly6C^lo/- F4-80⁺ bone marrow macrophages. Histograms show total numbers of macrophages and percent F4-80⁺ within Ly6C^hi monocytes (mean + SEM from seven mice per group). <b>(C)</b> Ly6C and CCR expression (MFI) on Ly6C^hi monocytes at day 28pi. <b>(D)</b> Percentage of CXCR4⁺, Sca-1⁺ and MHC-II⁺ cells within Ly6C^hi monocytes on day 28pi. See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006422#ppat.1006422.s008" target="_blank">S8 Fig</a>. <b>(E)</b> Percentage of Arginase-1 (Arg-1) and IL-10 expressing cells within Ly6C^hi monocytes. <b>(F)</b> NOS2 expression (MFI) on Ly6C^hi monocytes and Ly6C^lo/- F4-80⁺ bone marrow macrophages at day 28pi. <b>(G)</b> Parasite burden determined by the limiting dilution assay in WT and KO bone marrow at day 28. Data shown were pooled from two independent infections with 10 mice per genotype. <b>(H-I)</b> Pearson's correlation coefficient was used to assess correlation between bone marrow parasite burden and bone marrow HSCs <b>(H)</b> and Ly6C^hi monocytes <b>(I)</b> during the course of the infection. Data for correlation were pooled from C57BL/6, <i>Fzd6</i>^+/+ and <i>Fzd6</i>^-/- mice at day 14, 21 or 28. All bar graphs represent mean + SEM with 6 mice per group for day 28 and 3 mice per group for day 14 unless otherwise noted. Similar results were obtained from three independent experiments for day 28. *<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001.