Myeloid cells, particularly Ly6C^hi and Ly6C^lo/int monocytes, accumulate in the spleen of <i>L</i>. <i>donovani</i> infected mice over the course of infection.

<p>Mice were infected with <i>L</i>. <i>donovani</i> and sacrificed at various time points after infection. Neutrophils were excluded from all analysis involving monocytes. <b>(A)</b> Representative FACS plots depicting the gating strategy used to identify neutrophils (left) and percentage of neutrophils in the spleen of infected mice (right).<b>(B)</b> Gating strategy used to identify Ly6C⁺ monocytes (left) and percentage of splenic Ly6C^hi (upper graph) and Ly6C^lo/int (lower graph) monocytes. <b>(C)</b> Percentage of CD11c⁺ myeloid cells. <b>(D)</b> Percentage of CD11c⁺ Ly6C⁺ (left histogram raw and upper graph) and Ly6C^lo/int (right histogram raw and lower graph) monocytes. All data represent mean ± SEM of one of 4 independent experiments, n = 4.