CEACAM1 expression on splenic B and T cells changes only marginally during infection.

<p>Mice were infected i.n. with 5×10⁴ PFU of MHV-68. At days 0, 6, 17 and 42 after infection, CEACAM1 expression by spleen cells was determined by three color flow cytometry. The indicated cells were labeled either with the CEACAM1-specific mAb CC1 (open curves) or an isotype-matched antibody (filled curves). A) Granulocytes from <i>Ceacam1^−/−</i> and WT mice were gated according to their characteristic forward/sideward scatter (verified by anti-GR1 (Ly6G) staining) and analysed for CEACAM1 expression. B) CEACAM1 expression on lymphocyte subpopulations from WT mice at different time points after infection. Cells were gated for expression of CD19 (B cells; left panel) and for expression of CD3 (T cells; right panel). The mean fluorescence intensity (mfi) is indicated by numbers (mfi isotype control/mfi specific staining). The experiment was repeated three times with similar results.