T cell-intrinsic <i>Map3k8</i> does not contribute to exacerbated inflammation and pathology following <i>S</i>. <i>mansoni</i> infection.

<p><i>Cd4</i>^<i>Cre</i><i>Map3k8</i>^<i>+/+</i> and <i>Cd4</i>^<i>Cre</i><i>Map3k8</i>^<i>fl/fl</i> mice were infected percutenously with 50 <i>S</i>. <i>mansoni</i> cercariae and analysed at 8 weeks post-infection. A–C) Perfused tissue was fixed and embedded in paraffin before sectioning and staining with Masson’s trichrome. B) Granuloma size was determined from 10–20 individual granulomas per sample measured using Image J. D) Expression of <i>Col3</i> and <i>Col6</i> was determined from RNA extracted from liver or small intestinal tissue. Data is expressed relative to HPRT and shown as a fold-change relative to uninfected mice. E) Mesenteric lymph node cells were re-stimulated with anti-CD3 for 3 days. Cytokines were measured in supernatants, by ELISA. F) Naive T cells (CD4⁺CD44⁻CD25⁻CD62L⁺) were FACS purified from WT and <i>Map3k8</i>^{<i>–/–</i>}mice and cultured under T_H1 and T_H2 conditions. Frequencies of CD44⁺IFNγ⁺ and CD44⁺IL-4⁺ cells were determined by intracellular FACS analysis on day 7. All experiments are representative of 2–3 independent experiments with 5–10 mice/genotype. * p< 0.05 as assessed by two-tailed Mann-Whitney test.