<i>Map3k8</i>^–/–mice develop increased hepatic and intestinal inflammation and fibrosis following <i>S</i>. <i>mansoni</i> infection.

<p>WT and <i>Map3k8</i>^{<i>–/–</i>}mice were infected percutenously with 50 <i>S</i>. <i>mansoni</i> cercariae and analysed at 8 weeks post-infection. A & C) Perfused tissue was fixed and embedded in paraffin before sectioning and staining with Masson’s trichrome. B) Granuloma size was determined from 10–20 individual granulomas per sample measured using Image J. Scale bars are 1000μm (top), 200μm (middle) and 100μm (bottom). D) Intestinal pathology score, as described in methods. E) Expression of <i>Col3</i> and <i>Col6</i> was determined from RNA extracted from liver or small intestinal tissue. Data is expressed relative to HPRT. F) Hydroxyproline was quantified in liver tissue from naïve and infected animals. G) Frequency of T_REG (CD4⁺CD25⁺<i>Foxp3</i>^<i>RFP</i>+) and T_H2 (CD4⁺CD44⁺<i>Il4</i>^<i>GFP</i>+) cells in the spleen, mesenteric lymph nodes (MLN) and liver were determined by FACS. All experiments are representative of 2–3 independent experiments with 5–10 mice/genotype. * p< 0.05 as assessed by two-tailed Mann-Whitney test.