Inhibition of iN reprogramming by macrophages.

(A) Flow cytometry to quantify macrophage fractions with CD11b and F4/80 antibodies. (B) Temporal profiles of macrophage fractions in undepleted (top) and macrophage-depleted (bottom) MEFs during iN reprogramming. (C) Immunofluorescence staining of iNs with antibodies against βIII tubulin and CD11b on day 7. (D) Percentage of βIII tubulin (+) cells related to (C). (E) βIII tubulin (+) neurite area in each βIII tubulin (+) cell related to (C). The value with undepleted WT cells was defined as 1.0. (F) Immunofluorescence staining of iNs with βIII tubulin antibody on day 7 after mixing macrophage-depleted MEFs (before “+”) and macrophages (after “+”) of the 2 genotypes each. (G) Percentage of βIII tubulin (+) cells related to (F). (H) βIII tubulin (+) neurite area in each βIII tubulin (+) cell related to (F). * p p S1 Data. iN, induced neuron; MEF, mouse embryonic fibroblast; WT, wild-type.