Characterization of macrophage proliferation and maturation during iN reprogramming.

(A) PCR of macrophage genes upon culture of MEFs with and without BAM viruses. The values obtained with WT MEFs without virus on day 0 were defined as 1.0. (B) Flow cytometry of Per1-/- MEFs after culture in N3, N3 without ITS (N3, ITS (-)), and N3 without FGF2, progesterone, and putrescine (N3, FPP (-)) in the absence of viruses. (C) PCR of macrophages genes on day 10 after culture of MEFs under the conditions in (B). (D) MTS assays following the culture of purified macrophages in the N3 medium without BAM viruses. The values with WT cells on day 0 was defined as 1.0. (E) PCR of macrophage marker genes after culture of purified macrophages in the N3 medium without BAM viruses for 9 days. The values obtained with WT macrophages on day 0 were defined as 1.0. (F) ELISA of M-CSF in the supernatant during iN reprogramming. The culture medium was not replaced for 2 days before harvest. (G) MTS assays following the culture of purified macrophages in the N3 medium with 2 ng/ml M-CSF. The values with WT cells on day 0 was defined as 1.0. (H) PCR of macrophage marker genes after culture of purified macrophages in the N3 medium with 2 ng/ml M-CSF for 9 days. The values obtained with WT macrophages on day 0 were defined as 1.0. * p p S1 Data. (TIF)