Analyses of DNA replication and apoptosis during iN reprogramming.

(A) The frequency of EdU (+) cells during iN reprogramming. (B) Representative images of EdU uptake into macrophages (top) and non-macrophages (bottom). (C) The frequency of macrophages within the EdU (+) population during the reprogramming. (D) The frequency of EdU (+) cells within the macrophage population during the reprogramming. (E) TUNEL staining of undepleted WT cells during the reprogramming. (F) The frequency of TUNEL (+) cells in undepleted and macrophage-depleted cells during the reprogramming. There was no statistically significant difference between each genotype on day 10. Bar, 50 μm. * p p t test; ns indicates statistically not significant. All data were based on biological triplicates with technical triplicates each. The data underlying this figure can be found in S1 Data. (TIF)