Macrophages, but not DC, from the SI LP express TIM-4 at steady-state.

<p>APC were isolated from the SI of untreated mice and analysed for expression of cell surface molecules by flow cytometry. (A) Dot plots show the gating strategy employed to identify distinct SI DC and macrophage (Mφ) populations. (B) Histograms compare expression profiles of cell surface molecules and RALDH between DC and macrophage populations identified as in (A). (C) Intestinal macrophages were divided into TIM-4− and TIM-4+ populations and compared for expression of F4/80 and CX3CR1. Bar graphs show average median fluorescent intensity (MFI)±SEM for 3 individual mice. Data are from 1 of 3 independent experiments, each with 3 mice, that gave similar results.