Inhibition of iN reprogramming by macrophages.

(A) Flow cytometry to quantify the macrophage fractions in macrophage-depleted MEFs during iN reprogramming. (B) Immunofluorescence staining of iNs with MAP2 antibody on day 11. (C) Percentages of MAP2 (+) cells related to (B). (D) MAP2 (+) neurite area in each MAP2 (+) cell related to (B). The value with undepleted WT cells was defined as 1.0. (E) Flow cytometry to quantify purified macrophage fractions. (F) Percentages of purified macrophages on day 0. (G) Immunofluorescence staining of iNs with MAP2 antibody on day 11 after mixing macrophage-depleted MEFs (before “+”) and macrophages (after “+”) of 2 genotypes each. (H) Percentages of MAP2 (+) cells related to (G). (I) MAP2 (+) neurite area in each MAP2 (+) cell related to (G). * p p t test; ns indicates statistically not significant. All data are based on biological triplicates with technical triplicates each. The data underlying this figure can be found in S1 Data. (TIF)