UBE2C knockdown weakened proliferation, migration, and invasion.

(A) Morphological changes of SW-13 cells transfected with control or UBE2C siRNA. (B) SW-13 cells were transfected with control or UBE2C siRNA, and colonies were counted after 14 days. (C) SW-13 cells were transfected with control or UBE2C siRNA, and cell invasion was determined by transwell assay. (D) SW-13 cells were transfected with control or UBE2C siRNA, and cell migration was detected by wound healing assay. (E-F) Western blotting assay of the indicated proteins in SW-13 cells were transfected with UBE2C or control siRNA for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001, the asterisk represents the degree of statistical significance (*P) (n = 3).