Effect of α₂δ-1, Sp1, or TLR-4 silencing on the proliferation and migration of U87 cells.

A) Comparison of cell number after α2δ-1, Sp1, and TRL-4 knockdown with specific siRNAs of U87 cells as assessed by direct cell counting. Values are expressed as the percentage of untransfected cells (Ctl), and each bar represents the mean ± SEM of triplicate determinations in 3 separate experiments. *P versus untransfected cells. B) Transwell migration assays of control and cells transiently transfected with specific α2δ-1, Sp1, or TLR-4 siRNAs. Representative images from three separate experiments are shown. C) Comparative analysis of the mean ± SEM of 3 transwell independent experiments as in B. *P < 0.05 compared to untransfected cells.