Effect of α₂δ-1, Sp1, or TLR-4 overexpression on the proliferation and migration of U87 cells.

A) Comparison of cell number after transient transfection of U87 cells with α2δ-1 subunit, Sp1, and TRL-4 as assessed by direct cell counting. Values are expressed as the percentage of untransfected cells (Ctl), and each bar represents the mean ± SEM of triplicate determinations in 3 separate experiments. *P versus untransfected cells. B) Transwell migration assays of control and cells transiently transfected with α2δ-1, Sp1, or TLR-4 cDNA clones. Representative images from three independent experiments are shown. C) Comparative analysis of the mean ± SEM of 3 transwell separate experiments as in B. *P < 0.01 compared to untransfected cells.