Effect of α₂δ-1 overexpression on the proliferation and migration of U87 cells.

A) Western blot analysis of U87 cell lysates in control and α2δ-1 transiently transfected cells. The image in the upper panel shows a representative assay of three performed separately. The lower panel shows the densitometric analysis of the protein α2δ-1 normalized with respect to the expression of β actin and depicted as a fold change from the control. Asterisks denote a statistically significant difference (P 2δ-1 transfection in the presence and absence of the agonist and the antagonist of TLR-4, LPS, and C34, respectively. Values are expressed as the number of cells in each experimental condition, and each bar represents the mean ± SEM of triplicate determinations in 3 separate experiments. *P versus α2δ-1 overexpressing cells (second bar from the left). ⁋P versus untransfected cells. C) Transwell migration assays of U87 cells in the presence of the TLR-4 activator (LPS). The left panel shows representative images from three separate experiments. The right panel shows the comparative analysis of the mean ± SEM of 3 transwell independent experiments. *P < 0.01 compared to untreated cells.