UBE3C improves RCC cell proliferation, cell migration and invasiveness in vitro.

<p>(A) UBE3C expression of RCC cells infected with lentivirus. Left: Overexpression of UBE3C in 786-O cells. Right: Knockdown of UBE3C expression in ACHN cells. (B) The CCK-8 assay revealed that UBE3C overexpression significantly improved the cell growth rate (Left). Knockdown of UBE3C expression in ACHN cells significantly reduced the cell growth rate. Means±SD from three independent experiments were presented. (C). Left: Overexpression of UBE3C expression promoted cell colony-forming capacity in 786-O cells. Right: knockdown of UBE3C expression inhibited cell colony-forming capacity in ACHN cells. Means±SD from three independent experiments were presented. (D) Left: Overexpression of UBE3C expression promoted cell migration and invasion in 786-O cells. Right: Knockdown of UBE3C expression significantly inhibited cell migration and invasion in Matrigel transwell assays. The permeable cells were stained with Giemsa. Significant differences were determined using Student’s t test, *P<0.05, **P<0.01, ***P<0.001.