The effect of p130Cas knockdown on spreading and migration in Oli-<i>neu</i> does not result from changes in cellular viability.

<p>(A) Oli-<i>neu</i> cells were treated with p130Cas or control siRNA. 24 hours later, the cells were detached from their culture vessel and 6 h after re-plating a TUNEL assay was performed to test for apoptosis. Data show the mean ± SEM from 3 independent experiments; ns, not significant (Student’s <i>t</i> test). (B) Knockdown of p130Cas in Oli-<i>neu</i> cells. The siRNA-treated cells as analyzed in A were lysed and p130Cas levels were assessed by Western blotting. α-tubulin serves as loading control. (C) Oli-<i>neu</i> cells were treated with p130Cas or control siRNA. 24 h later, the cells were detached from their culture vessel and 0.5 h and 6 h after re-plating MTT and LDH assays were carried out to test for cell viability and membrane integrity, respectively. Data show the mean ± SEM from 3 independent experiments; ns, not significant (Student’s <i>t</i> test). (D) Knockdown of p130Cas in Oli-<i>neu</i> cells. The siRNA-treated cells as analyzed in C were lysed and p130Cas levels were assessed by Western blotting. α-tubulin serves as loading control.