Additional file 3 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Additional file 3: Figure sup 3. Similar levels of astrogliosis were observed in apoE-TR mice upon cuprizone-induced demyelination. The astrogliosis in the CC area of CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice was examined by immunostaining for GFAP. (A) Representative images of GFAP+astrocytes in the CC area of apoE-TR mice are shown. Scale bar, 100 µm. (B) The morphology of GFAP+astrocytes in apoE-TR mice are shown. Scale bar, 25 µm. (C) The fold change of GFAP+ staining in CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice was quantified. (D) The number of GFAP+ astrocyte per high-power field (HPF; 3 HPFs/mouse) was analyzed. (E) The surface area of GFAP+ astrocyte was quantified. Values are mean ± SEM. Two-way ANOVA. N.S., not significant