Additional file 1 of β-Arrestin2-biased Drd2 agonist UNC9995 alleviates astrocyte inflammatory injury via interaction between β-arrestin2 and STAT3 in mouse model of depression

Additional file 1: Fig. S1. CUMS depression model induces astrocyte dysfunction in the hippocampus of mice. (A) Astrocyte dysfunction induced by CUMS. Representative images of astrocyte immunostaining for GFAP in mice hippocampus, Scale bar: 200 μm (upper panel); Scale bar: 40 μm (lower panel). (B) Immunofluorescence staining of NeuN in mice hippocampus. NeuN: Red; DAPI: Blue; Scale bar: 100 μm. (C) Microglial activation induced in CUMS model. Representative images of microglial immunostaining for Iba-1 in mice hippocampus, Scale bar: 100 μm. (D) The protein level of GFAP was detected in the hippocampus of mice in CUMS model. (E) Immunoblot analysis and quantification of GFAP (n = 3). (F) Relative mRNA expression of IL-1α, IL-1β, IL-6, and TNF-α in the hippocampus of mice in CUMS model (n = 3). Data were presented as mean ± SEM; statistically significant by Student t-test; *P 0.05, *P 0.05, *P 0.05, *P 0.05, #P 0.05, $P 0.05, *P 0.05, *P 0.05, *P 0.05, #P 0.05, $P < 0.05, $$$P < 0.001 VS WT CORT+UNC9995 group. Supplementary Table 1. Primers used in the study