TMPRSS6 isoforms engage in homo- and hetero-interactions.
- Sébastien P. Dion (4220848)
- Antoine Désilets (4220842)
- Gabriel Lemieux (13549950)
- Richard Leduc (1279377)
Publication date
August 2022
DOI Publisher
Public Library of Science (PLoS)Abstract
HEK293 cells were co-transfected with V5- and HA-tagged TMPRSS6 isoforms. Homo- and hetero-interaction of TMPRSS6 isoforms were assessed by immunoprecipitation (IP) of cell lysate (CL) using an anti-V5 antibody. Isoform immunoprecipitation was detected using anti-V5 and anti-HA antibodies. Equal amount of CL was loaded on SDS-polyacrylamide gels and cell GAPDH was used as a loading control (n = 3).</p
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