<p>(A) Sequence alignment of the N-termini of TMPRSS2 isoforms 1 and 2. Identical amino acids are marked with stars. Amino acids absent in isoform 2 are marked with ‘–‘. (B) Plasmids encoding TMPRSS2 isoform 1 and isoform 2, both equipped with an N-terminal myc tag, were transiently transfected into 293T cells. Empty plasmid (pCAGGS) served as a negative control. Protease expression in cell lysates was detected via Western blotting with anti-myc antibody. The β-actin expression served as a loading control. Black-filled arrowheads indicate the zymogen forms, while grey-filled arrowheads highlight cleavage products resulting from protease activation. (C) 293T cells were transfected as described for panel B but protease expression was determin...
<p>(A) Western blots were performed on two different membrane fractions of HEK-293 cells transfected...
A. Transfection of pgMAX/DsRed2 (mammalian mode). Transfected plasmids are indicated. Phase-contrast...
<p>(A) TgRRM1 was immunoprecipitated with anti-myc antibody from the whole cell lysates (shown here)...
<p>(A) Expression plasmids encoding FLUAV HA subtypes H1 (left) and H3 (right) and the indicated pro...
<p>(A) COS-7 cells were transfected with plasmids encoding TMPRSS2 isoform 1 or isoform 2 or with em...
The cellular protease TMPRSS2 cleaves and activates the influenza virus hemagglutinin (HA) and TMPRS...
<p><b>A.</b> Subcellular localization of HA, p24 and TMPRSS2 proteins in transfected HEK 293T cells ...
<p>(A) Expression plasmids encoding the HA of the 1918 influenza virus and the indicated proteases o...
HEK293 cells were co-transfected with V5- and HA-tagged TMPRSS6 isoforms. Homo- and hetero-interacti...
<p>(A) HEK293T cells were transfected with FLAG-tagged TMCC1 plasmid or the vector; 24 h post-transf...
<p>HEK-293T cells were co-transfected with expression vectors encoding myc-LRIG1 or FLAG-LRIG2 and P...
<p>HEK293 cells were transfected with expression plasmids for wild-type (WT) or glycosylation mutant...
A) TfR1-HA was transfected with one or two TMPRSS6-V5 tagged isoforms. Expression was detected in th...
<p>(A) Lysates of cells, which were co-transfected with Core-p7, FLAG-NS2, and SPCS1-myc expression ...
<p>Ost TK1<sup>−</sup> cells were transiently transfected with FLAG-pCMV2 plasmids coding for TK1 va...
<p>(A) Western blots were performed on two different membrane fractions of HEK-293 cells transfected...
A. Transfection of pgMAX/DsRed2 (mammalian mode). Transfected plasmids are indicated. Phase-contrast...
<p>(A) TgRRM1 was immunoprecipitated with anti-myc antibody from the whole cell lysates (shown here)...
<p>(A) Expression plasmids encoding FLUAV HA subtypes H1 (left) and H3 (right) and the indicated pro...
<p>(A) COS-7 cells were transfected with plasmids encoding TMPRSS2 isoform 1 or isoform 2 or with em...
The cellular protease TMPRSS2 cleaves and activates the influenza virus hemagglutinin (HA) and TMPRS...
<p><b>A.</b> Subcellular localization of HA, p24 and TMPRSS2 proteins in transfected HEK 293T cells ...
<p>(A) Expression plasmids encoding the HA of the 1918 influenza virus and the indicated proteases o...
HEK293 cells were co-transfected with V5- and HA-tagged TMPRSS6 isoforms. Homo- and hetero-interacti...
<p>(A) HEK293T cells were transfected with FLAG-tagged TMCC1 plasmid or the vector; 24 h post-transf...
<p>HEK-293T cells were co-transfected with expression vectors encoding myc-LRIG1 or FLAG-LRIG2 and P...
<p>HEK293 cells were transfected with expression plasmids for wild-type (WT) or glycosylation mutant...
A) TfR1-HA was transfected with one or two TMPRSS6-V5 tagged isoforms. Expression was detected in th...
<p>(A) Lysates of cells, which were co-transfected with Core-p7, FLAG-NS2, and SPCS1-myc expression ...
<p>Ost TK1<sup>−</sup> cells were transiently transfected with FLAG-pCMV2 plasmids coding for TK1 va...
<p>(A) Western blots were performed on two different membrane fractions of HEK-293 cells transfected...
A. Transfection of pgMAX/DsRed2 (mammalian mode). Transfected plasmids are indicated. Phase-contrast...
<p>(A) TgRRM1 was immunoprecipitated with anti-myc antibody from the whole cell lysates (shown here)...