Murine and rat IGSF1-2 proteins are not secreted from transfected cells.

<p>HEK293 cells were transfected with expression plasmids for wild-type (WT) or glycosylation mutant (NQ) forms of murine or rat IGSF1-2, murine transthyretin (TTR), or empty vector (pcDNA4). Note, in all cases, proteins were expressed with Myc/His tags at their C-termini. Media (top two panels) and whole cell protein lysates (bottom panel) were collected and analyzed by SDS-PAGE and immunoblotting for Myc. In the middle panel, proteins in culture medium were analyzed directly. In the top panel, proteins in the media were analyzed following Ni-NTA purification and enrichment.