A) TfR1-HA was transfected with one or two TMPRSS6-V5 tagged isoforms. Expression was detected in the cell lysate (CL) and cell media (CM) (n = 3). B) TfR1 cleavage quantification. Results are presented as TFR1 cleavage (%) relative to TFR1 cleavage by TMPRSS6 isoform 2. Statistical significance was assessed using one sample T test, *p < 0.02. The means ± SD are presented (n = 3).</p
<p>(A) Schematic representation of WT or truncated-TLR11-Flag. Numbers represent the initial amino a...
(A) Measure of STAT6 mRNA level in HT-29 cells 24 hours post-transfection. The graph represents the ...
<p>(<b>A</b>) BHK cells were transfected with 1.0 µg HA plasmid and 0.25 µg of either HAT or TMPRSS2...
TfR1-HA was transfected either with V5-tagged TMPRSS6 isoform 2 WT (active, TMPRSS6-2-WT-V5), cataly...
HEK293 cells were co-transfected with V5- and HA-tagged TMPRSS6 isoforms. Homo- and hetero-interacti...
<p>(A) Sequence alignment of the N-termini of TMPRSS2 isoforms 1 and 2. Identical amino acids are ma...
A) BioVenn overlap of TMPRSS6 isoforms 2, 3 and 4 interacting partners (fold change vs mock ≥ 3) ide...
<p>Hepa1-6 cells stably co-expressing FLAG-tagged Hfe and myc-tagged Tfr2 (WT), or truncated Tfr2 (Y...
<p>(A) The cells plated on coverslips were transfected with 100ng of GFP-E50K alone or 200ng of TFR ...
<p>A. Immunoprecipitation of TSAP6 N-terminal fragments. The cell lysate of HEK-293T cells transfect...
<p>RNA purified from CTLMC and MLMC was subjected to quantitative real-time PCR using SYBR Green for...
<p>(A) Immunofluorescence microscopy of resting (top) or imiquimod activated (bottom) fibroblast co-...
<p>(A) Liver nucleic lysates from mice were analyzed by Western blot for p-Stat5 relative to TBP. Va...
<p><i>Bmp6</i> (<b>A, C, E</b>) and <i>Tfr1</i> (<b>B, D, F</b>) expression was measured in liver ce...
(A) Schematic diagram of N-terminal Myc-tagged and C-terminal HA-tagged MYRF constructs used for co-...
<p>(A) Schematic representation of WT or truncated-TLR11-Flag. Numbers represent the initial amino a...
(A) Measure of STAT6 mRNA level in HT-29 cells 24 hours post-transfection. The graph represents the ...
<p>(<b>A</b>) BHK cells were transfected with 1.0 µg HA plasmid and 0.25 µg of either HAT or TMPRSS2...
TfR1-HA was transfected either with V5-tagged TMPRSS6 isoform 2 WT (active, TMPRSS6-2-WT-V5), cataly...
HEK293 cells were co-transfected with V5- and HA-tagged TMPRSS6 isoforms. Homo- and hetero-interacti...
<p>(A) Sequence alignment of the N-termini of TMPRSS2 isoforms 1 and 2. Identical amino acids are ma...
A) BioVenn overlap of TMPRSS6 isoforms 2, 3 and 4 interacting partners (fold change vs mock ≥ 3) ide...
<p>Hepa1-6 cells stably co-expressing FLAG-tagged Hfe and myc-tagged Tfr2 (WT), or truncated Tfr2 (Y...
<p>(A) The cells plated on coverslips were transfected with 100ng of GFP-E50K alone or 200ng of TFR ...
<p>A. Immunoprecipitation of TSAP6 N-terminal fragments. The cell lysate of HEK-293T cells transfect...
<p>RNA purified from CTLMC and MLMC was subjected to quantitative real-time PCR using SYBR Green for...
<p>(A) Immunofluorescence microscopy of resting (top) or imiquimod activated (bottom) fibroblast co-...
<p>(A) Liver nucleic lysates from mice were analyzed by Western blot for p-Stat5 relative to TBP. Va...
<p><i>Bmp6</i> (<b>A, C, E</b>) and <i>Tfr1</i> (<b>B, D, F</b>) expression was measured in liver ce...
(A) Schematic diagram of N-terminal Myc-tagged and C-terminal HA-tagged MYRF constructs used for co-...
<p>(A) Schematic representation of WT or truncated-TLR11-Flag. Numbers represent the initial amino a...
(A) Measure of STAT6 mRNA level in HT-29 cells 24 hours post-transfection. The graph represents the ...
<p>(<b>A</b>) BHK cells were transfected with 1.0 µg HA plasmid and 0.25 µg of either HAT or TMPRSS2...