Atypical PKCζ exists in a complex with HDAC6.

<p>HA-PKCζ and FLAG-HDAC6 were transfected into HEK cells and reciprocal immunoprecipitations performed using antibodies for each tag as described in Materials and Methods. Control lanes (C) were transfected with empty plasmid while sample lanes (T) were transfected with both HA-PKCζ and FLAG-HDAC6. 40 μg of whole cell lystate (Lysate) was loaded to show construct expression in transfected cells. Co-precipitating bands were detected by SDS-PAGE and Western blot with tag-specific antibodies. Results are indicative of 3 independent experiments.