Immunodetection of heterologously expressed constructs.

<p>Immunodetection of flag-tagged KCNE1 constructs. <b>A</b>, crude membrane preparations from HEK cells transiently transfected with respective KCNE1 constructs. Actin (∼42 kD) is shown as loading control. <b>B</b>, effective co-immunoprecipitation (IP) occurred for Kv7.1 with all flag-tagged KCNE1 constructs. The upper blot shows protein samples from HEK cells precipitated by anti-flag and bands detected by anti-Kv7.1. The lower blot shows samples precipitated by anti-Kv7.1 and bands detected by anti-flag antibody (n = 2 experiments each). <b>C</b> shows respective confocal images of KCNE1 subunits expressed without Kv7.1. Bars represent 5 µm. Images are representative of at least 5 different experiments. NT – non-transfected control, IP – immunoprecipitation.