Characterization of Jc1^Flag-E2 and JFH1^Flag-E2 infectious HCVcc constructs.

<p>(<b>A</b>) Scheme of the JFH1^Flag-E2 and Jc1^Flag-E2 constructs used in this study. (<b>B</b>) Western blot analysis of Huh7.5 cells transfected with <i>in vitro</i> transcribed JFH1^Flag-E2 and Jc1^Flag-E2 RNA with anti-Flag, anti-E2, anti-core, anti-actin antibodies. (<b>C</b>) TCID₅₀ of Huh7.5 cells transfected with <i>in vitro</i> transcribed JFH1^Flag-E2 and Jc1^Flag-E2 RNA. (<b>D</b>) Confocal microscopy of Huh7 Lunet cells infected with JFH1^Flag-E2 and Jc1^Flag-E2 viral stocks after immunostaining with antibodies recognizing E2 and Flag. Single channels are shown in black and white; the merged image is pseudocolored with ADRP in red and LipidTox in blue (scale bars 5 µm).