KCNE1 mutations D76N and Δ70 reduce I<sub>Ks</sub> current density with minimal effects on activation kinetics.

Jerri Chen (373652)
Renjian Zheng (373656)
Yonathan F. Melman (373660)
Thomas V. McDonald (34719)

Publication date

February 2013

DOI

10.1371/journal.pone.0005143.g002

Abstract

<p>A) rates of activation, and B) current density from CHO cells transiently expressing Myc-tagged KCNQ1 (Q1) either without KCNE1 (E1), or with wild-type FLAG-tagged E1, E1-D76N, or E1-Δ70. Asterisks (*) indicate statistical significance to P<0.001. n = 6. C) Immunoblot showing KCNQ1 protein levels when expressed alone or co-expressed with KCNE1 (wild-type and mutant). Western blot analysis performed with mouse anti-Myc antibody (Santa Cruz) for KCNQ1, and mouse anti-FLAG antibody (Sigma) for KCNE1. Left lane in each gel shows signal from cells that are untransfected.</p

Extracted data

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KCNE1 mutations D76N and Δ70 reduce I<sub>Ks</sub> current density with minimal effects on activation kinetics.

Abstract

Extracted data

KCNE1 mutations D76N and Δ70 reduce I<sub>Ks</sub> current density with minimal effects on activation kinetics.

Abstract

Extracted data

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